Journal: Scientific Reports

Article Title: A high-throughput pipeline for scalable kit-free RNA extraction

doi: 10.1038/s41598-021-02742-w

Figure Lengend Snippet: SARS-CoV-2 detection on hypothetical patient samples. Oropharyngeal nasal mid-turbinate samples were collected from 24 volunteers and spiked 8 each with 1 × 10 4 copies/ml (blue square, blue circle), 1 × 10 5 copies/ml (red square, red circle), heat inactivated 2019 novel coronavirus (nCoV), or water (green square, green circle). Total RNA was extracted using Opentron-2 (OT-2; squares ) and Eppendorf epMotion (epMotion; circles ). ( A ) Biological samples were analysed by SYBR one-step RT-qPCR targeting SARS-CoV-2 N gene. Artefact amplification was observed in water-spiked samples (green square, green circle). ( B ) Biological samples were analysed by multiplex, probe-based RT-qPCR targeting SARS-CoV-2 ORF1a gene.

Article Snippet: Automated pipetting was performed using an 8-channel pipetting arm of the OT-2 lab robot (OpenTrons, USA) and epMotion 5075 (Eppendorf, Germany).

Techniques: Quantitative RT-PCR, Amplification, Multiplex Assay